Figure 2
Sorting strategy for the isolation of CD34+CD146+CD31+CD45− cells from spleen-derived MNCs. Splenic MNCs were first enriched in CD34+ cells by immunomagnetic selection, followed by staining with anti-CD146, anti-CD31, and anti-CD45 mAbs, and then sorted according to CD34, CD31, and CD146 positive and CD45 negative expression. Contaminating platelets were excluded by size gating.

Sorting strategy for the isolation of CD34+CD146+CD31+CD45 cells from spleen-derived MNCs. Splenic MNCs were first enriched in CD34+ cells by immunomagnetic selection, followed by staining with anti-CD146, anti-CD31, and anti-CD45 mAbs, and then sorted according to CD34, CD31, and CD146 positive and CD45 negative expression. Contaminating platelets were excluded by size gating.

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