GEP upon SOX11 silencing. (A) SOX11 silencing in Z138 stably transduced with shSOX11.1, shSOX11.2, and shSOX11.3 was verified (upper panel) at the protein level by WB using GAPDH as a loading control and (lower panel) at the transcriptional level by qRT-PCR. A scramble shControl and untransduced cells (Z138) are also shown. Bar plot represents the mean ± SD of 3 independent experiments. (B) Heat map illustrating 366 significant differentially expressed genes (adjusted P < .05 and log2 FC > 0.7) in Z138 shSOX11.1 and shSOX11.3 compared with 2 Z138-shControl cells. Red indicates increased expression and green decreased expression relative to the median expression level according to the color scale shown. (C) DAVID functional annotation of the 366 differentially expressed genes. The 11 most significant (P < .05) biological process GO terms, their P value, enrichment score, and gene count are shown. (D) Venn diagram depicting the overlap of 1133 SOX11-bound genes (orange circle), 2790 significant differentially expressed genes (P < .05) upon SOX11 silencing (blue circle), and differentially expressed genes using more stringent statistical criteria (P < .05 and log2 FC > 0.7) (purple circle).