Raptor-deficient DCs express less CCR7. (A) Cells from control mice were left to migrate into medium containing 0, 100, 200, or 500 ng/mL rapamycin. Populations in plots are gated on CD45⁺ cells. The mean frequency ± SEM of CD11c⁺ MHC-II⁺ cells is indicated below the gate and in the bar graph on the right (N = 3 mice per group). The histogram below shows the expression of CCR7 by DCs gated as shown in the top panel without or with 500 ng/mL rapamycin. The bar graph on the right represents the MFI ± SEM of CCR7 with increasing concentrations of rapamycin. (B) gated as shown in panel A. Histograms show the expression of CCR7 by skin derived DCs (top panel) or by CD207⁺ DCs (bottom panel) isolated by crawl-out from whole skin explants of Cre (black line), rap^Δ/Δ (red line), and ric^Δ/Δ (green line) mice. Bar graphs represent the MFI ± SEM of N = 2 mice per group. One representative of 2 independent experiments with similar results is shown.