P110α expression does not increase with relapse in follicular lymphoma and gene expression of PIK3CA is significantly higher in MCL compared with indolent NHL and CLL. (A) Gene expression of class IA PI3K isoforms at diagnosis in MCL was compared with indolent NHL using publicly available Affymetrix data (European Bioinformatics Institute, www.ebi.ac.uk ref: E-GEOD-16455) with the web-based software O-miner. cMCL, conventional MCL, CLL, chronic lymphocytic leukemia, FL, follicular lymphoma; HCL, hairy cell leukemia, SMZL, splenic marginal zone lymphoma. PIK3CA expression is significantly higher in MCL compared with indolent NHLs (Benjamini-Hochberg multiple testing adjusted P values: cMCL vs HCL = .005, cMCL vs SMZL = .0001 cMCL vs CLL = .003, cMCl vs FL = .05) in this study, whereas PIK3CB and PIK3CD expression is not. (*P < .05, **P < .01, ***P < .001). (B) IHC images of 4 follicular lymphoma diagnosis: second-relapse pairs showing no increase in p110α expression with relapse (original magnification ×200). (C) Dot plot comparing expression of p110α in sequential biopsies from follicular lymphoma patients (n = 10). No evidence of increased p110α expression is seen with relapse. Pairs are connected by straight lines.