Binding of VWF mutants to FVIII, GPIb and GPIIbIIIa. Recombinant VWF, wt (▪), p.D2509G (□), or p.L1696R (○), and p.P1824H (△) produced in BHK cells were tested for binding to FVIII (A), GPIIbIIIa (B), wt-GPIb in the presence of botrocetin (C), or to a GPIb carrying p.D235Y/M239V mutations (D) as described in “Materials and methods.” Data represent means ± SD of 3 independent experiments, except for binding to FVIII, where means ± SEM are represented. *P values < .05 as compared with wt values. OD, optical density; NP, normal pooled plasma.