Multimer analysis of patients heterozygous for the p.L1696R or p.P1824H mutation. VWF multimeric pattern from plasma samples of patients and affected family members. A sample from F2 I-1 was not available for analysis. For 2 individuals (F1 I-1 and F2 I-2), samples were analyzed twice using a 1:1 (indicated as 15% Ag a) or 1:2 dilution (indicated as 15% Ag b). Samples of patients F1 II-1 and F1 II-2 were diluted 1:2, whereas plasma of patient F2 I-3 was diluted 1:1. To demonstrate the role of sample dilution on multimer quality, VWF-deficient plasma was mixed with normal pooled plasma to a final concentration of 15% VWF:Ag. This preparation was subject to multimer analysis using a 1:1 (15% Ag a) or a 1:2 (15% Ag b) dilution. NP, normal pooled plasma.