American Society of Hematology

Figure 4

$Figure 4. Cvx-/Thr-induced PS exposure is absent in Scott syndrome platelets despite normal cyclophilin D-dependent mitochondrial depolarization. (A-D) Platelets from healthy controls or a Scott patient were loaded with TMRE and preincubated with vehicle (dimethylsulfoxide) or cyclosporin A (CsA) (4 μM) for 10 min prior to stimulation by Cvx/Thr (70 ng/mL, 4 nM, for 15 min) in the presence of 2 mM CaCl2. Graphs show annexin (Anx) A5 binding (A) and loss of TMRE (B) of washed control and Scott syndrome platelets. (C-D) Representative histogram and bar graph showing the distribution of Cvx-/Thr-stimulated control platelets (15 min) over the fractions M1, M2, and M3. (E-F) TMRE-loaded Scott platelets adhered to fibrinogen (10 min) were stimulated with Cvx/Thr (70 ng/mL, 4 nM) or ionomycin (10 μM) in the presence of 2 mM CaCl2. (E) Representative images after 15-min stimulation, (F) The percentage of TMRE-negative platelets. Mean ± SEM (n = 3-6); *P < .05.$

Cvx-/Thr-induced PS exposure is absent in Scott syndrome platelets despite normal cyclophilin D-dependent mitochondrial depolarization. (A-D) Platelets from healthy controls or a Scott patient were loaded with TMRE and preincubated with vehicle (dimethylsulfoxide) or cyclosporin A (CsA) (4 μM) for 10 min prior to stimulation by Cvx/Thr (70 ng/mL, 4 nM, for 15 min) in the presence of 2 mM CaCl₂. Graphs show annexin (Anx) A5 binding (A) and loss of TMRE (B) of washed control and Scott syndrome platelets. (C-D) Representative histogram and bar graph showing the distribution of Cvx-/Thr-stimulated control platelets (15 min) over the fractions M1, M2, and M3. (E-F) TMRE-loaded Scott platelets adhered to fibrinogen (10 min) were stimulated with Cvx/Thr (70 ng/mL, 4 nM) or ionomycin (10 μM) in the presence of 2 mM CaCl₂. (E) Representative images after 15-min stimulation, (F) The percentage of TMRE-negative platelets. Mean ± SEM (n = 3-6); *P < .05.

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