Kinetics and characterization of WT1-CTL. Monitoring the emergence of WT1-CTL post-DLI in UPN7. (A) Absolute number of WT1-CTL determined by HLA-A*0201/RMF MHC-tetramer staining. (B) Frequency and phenotypes of PB and BM-resident WT1-CTL in UPN7 from time point indicated by “FACS” arrow in panel C. Effector memory T cells dominate the PB, whereas BM-resident T cells are predominantly central memory in phenotype. (C) Longitudinal phenotypic analysis of WT1-CTL over the course of the disease in UPN7. T_N indicates naive T cells; T_E, effector T cells; T_EM, effector memory T cells; and T_CM, central memory T cells.