Figure 7
![Figure 7. The effect of Pep1 on the TG-2–mediated incorporation of biotinamido-pentylamine to fibrin TG-2 (11 µg/mL) was added to fibrin-coated wells followed by increasing concentrations of Pep1 (10-100 µM). A reaction mix containing DTT (0.1 mM), biotinamido-pentylamine (0.3 mM), CaCl2 (10 mM), and human α-thrombin (1 U/mL) in 100 mM 2-[Bisamino]-2–1,3-propanediol, pH 7.4, 0.0% triton X-100 was added to each well, and TG-2 activity was taken after 7 minutes. Samples were performed in triplicate (n = 3), and the results are expressed as means (SEM).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/121/11/10.1182_blood-2012-07-446393/3/2117f7.jpeg?Expires=1724227266&Signature=eiERPZajlJFF9m01VF8ZtZ7roh5eit~Szpn-XrPt6cJ5~gPvSAqauxifwmSMIELtmwJtrUByfMVfJQUWRYxM~zU5Bftu8XXVmLyY~56S4U50KwZDm1ZZP04H~d~jbAnkCYjR00AdT8nmDRmNFu-2yCY2QD701UfCunz-nlaBLL19IVfdfVrMR1WmS8UllrV0T0XJN4KCMEQdNrz0SxEAR4b7OcQu0Cvvnk17qf8MfXHxUEAgAswoWSv3MbWq0tHKsIV7HXOzPRiKvnPIONGAAlRj465wxxorD4C2Va0Fn8N~nsQJqKHeQWYkJDNBQqLdp8t4vWq22E6LYhcTzcbWMw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The effect of Pep1 on the TG-2–mediated incorporation of biotinamido-pentylamine to fibrin TG-2 (11 µg/mL) was added to fibrin-coated wells followed by increasing concentrations of Pep1 (10-100 µM). A reaction mix containing DTT (0.1 mM), biotinamido-pentylamine (0.3 mM), CaCl2 (10 mM), and human α-thrombin (1 U/mL) in 100 mM 2-[Bisamino]-2–1,3-propanediol, pH 7.4, 0.0% triton X-100 was added to each well, and TG-2 activity was taken after 7 minutes. Samples were performed in triplicate (n = 3), and the results are expressed as means (SEM).
