CAR-mediated induction of CYP2B6 and CYP3A4 in human primary hepatocytes. CYP2B6 and CYP3A4 mRNA and protein were measured in human primary hepatocytes prepared from 4 donors treated with CITCO (1μM), RIF (10μM), or vehicle control (0.1% DMSO) as outlined in “Methods.” (A-B) Induction of CYP2B6 and CYP3A4 mRNA analyzed with RT-PCR in HPHs from liver donors (HL#23, HL#24, HL#35, and HL#37). (C) Representative immunoblots of CYP2B6 and CYP3A4 proteins in HPHs from liver donors (HL#23, HL#24, and HL#37). RT-PCR data obtained from 3 independent experiments were expressed as mean ± SD normalized against vehicle control.