KSHV-related pathologies display high level of D6 expression. (Ai) KS tissue samples were stained for D6 immunoreactivity. D6 positivity is represented by the brown coloration and is seen throughout the tumor mass. (Aii) Staining for D6 immunoreactivity in healthy human skin confirming the specificity of staining by the antibody (basal keratinocyte staining indicated by arrows). (B) Costaining of a KS lesion for expression of D6, Lyve1, and podoplanin. Insets show (Bi) D6 (fluorescein isothiocyanate; green), (Bii) Lyve 1 (Texas Red; red), and (Biii) podoplanin (Alexa-647; purple). Complete overlap among all 3 markers leads to a pink coloration. (C) QPCR analysis of expression of vIL-6 in control and PEL cell lines. EBV and KSHV status is indicated below the graph. (D) QPCR analysis of expression of D6 in control and PEL cell lines. Again, EBV and KSHV status is indicated below the graph. (E) QPCR analysis of vIL-6 induction by phorbol ester at the indicated concentrations of TPA. (F) QPCR analysis of D6 expression in cell lines after phorbol ester treatment at the indicated concentrations of TPA.