PLSCR1 is involved in wogonoside-induced differentiation. U937 and HL-60 cells were transfected with nonspecific siRNA and PLSCR1 siRNA treated with or without 150 μM wogonoside for 96 hours. (A) Confirmation of the silencing of PLSCR1 expression was detected by western blotting with β-actin as a loading control. (B) The percentages of cells in G₀/G₁ phases of the cell cycle are shown. (C) Detection of NBT-reduction activity is shown. (D) CD11b-/CD14-positive cells were measured by flow cytometry. (E) Shown are the effects of silencing PLSCR1 on the expression of cell cycle– and differentiation-related proteins, which could be influenced by wogonoside. These figures are representative of 3 separate experiments.