Wogonoside increases PLSCR1 transcription and influences the expression of cell cycle– and differentiation-related proteins. (A) Total RNAs were extracted at the indicated time points. PLSCR1 mRNA levels were detected by quantitative real-time reverse transcription-PCR, and fold changes were assessed and shown normalized to GAPDH (glyceraldehyde-3-phosphate dehydrogenase) mRNA level. For analysis of RT-PCR results, asterisks denote significant (P < .05) differences relative to controls by 2-tailed Student t tests. (B) Wogonoside induced PLSCR1 expression. Whole cell extracts were analyzed by western blotting for PLSCR1 protein, using β-actin as a loading control. Cells treated with ATRA for 96 hours were used as a positive control. (C) Cells were cultured for 48 hours with or without 150 μM wogonoside after a 4-hour preincubation period with 4 μg/mL actinomycin D, and then analyzed for PLSCR1 and β-actin protein expression by western blotting. (D) The effects of wogonoside on the expression of p21cip and c-Myc proteins are shown.