Effects of dmPGE₂ and the EP4 agonist on the expression of niche-related genes in ALCAM^–Sca-1⁺ MPCs. ALCAM^–Sca-1⁺ cells were isolated and cultured on type 1 collagen–coated plates (BD Biocoat) (7000 cells/well) in MSCBM (Lonza). After 1 day of culture, cells were incubated with dmPGE₂ (10 μM), the EP4 agonist (0.1, 1, and 10 μM), or ethanol at 37°C overnight. RNA was isolated and reverse transcribed, and qPCR array analysis was performed. (A) Cytokine-related genes: Angpt1, Angptl2, Angptl4, Bmp2, Bmp6, Cxcl12, Fgf1, Fgf2, Flt3l, Igf1, Igf2, Il6, Dll1, Jag1, Kitl, Lif, Nov, Tgfb1, Vegfa, and Wnt5a. (B) Cell adhesion- and extracellular matrix–related genes: Alcam, Fn1, Has1, Has2, Tnc, and Spp1. (C) Proteolytic enzyme–related genes: Mmp2. ND, not detected.