American Society of Hematology

Figure 1

$Figure 1. Expression of EP receptors in hematopoietic cells. (A) qPCR analysis of Ep2, Ep3, and Ep4 mRNA expression in Lin+, Lin–, LSKCD34+Flt3+, LSKCD34+Flt3–, and LSKCD34–Flt3– fractions. Data represent means ± SD (*P < .05, n = 4). Representative data from 4 independent experiments are shown. Ep1 expression was not detected in hematopoietic cells. (B) Copy number determination of Ep2, Ep3, and Ep4 in LSKCD34+Flt3+, LSKCD34+Flt3–, and LSKCD34–Flt3– cells (250 cells/sample). The numbers of estimated target genes were analyzed by Digital Array. More Ep4-positive signals were observed in LSKCD34+Flt3+ and LSKCD34–Flt3– cells than Ep2- and Ep3-positive signals. Data represent means ± SD (*P < .01, n = 3). Representative data from 2 independent experiments are shown. ND, not detected.$

Expression of EP receptors in hematopoietic cells. (A) qPCR analysis of Ep2, Ep3, and Ep4 mRNA expression in Lin⁺, Lin^–, LSKCD34⁺Flt3⁺, LSKCD34⁺Flt3^–, and LSKCD34^–Flt3^– fractions. Data represent means ± SD (*P < .05, n = 4). Representative data from 4 independent experiments are shown. Ep1 expression was not detected in hematopoietic cells. (B) Copy number determination of Ep2, Ep3, and Ep4 in LSKCD34⁺Flt3⁺, LSKCD34⁺Flt3^–, and LSKCD34^–Flt3^– cells (250 cells/sample). The numbers of estimated target genes were analyzed by Digital Array. More Ep4-positive signals were observed in LSKCD34⁺Flt3⁺ and LSKCD34^–Flt3^– cells than Ep2- and Ep3-positive signals. Data represent means ± SD (*P < .01, n = 3). Representative data from 2 independent experiments are shown. ND, not detected.

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