Wnt signaling stimulates proplatelet production in fetal liver MKsex vivo. (A) Percentage of cultured MKs extending proplatelets in cells treated with Wnt3a, Wnt5a, and DKK1. Data are presented as mean ± SD from 3 individual experiments. P values and significance have been determined by 1-way ANOVA followed by Dunnett posttest to compare all groups with untreated cells. (B-D) Representative images of proplatelet-producing MKs in untreated, Wnt3a, and Wnt5a treated samples, respectively.