Ex vivo analysis of fetal liver megakaryopoiesis in LRP6^−/− mice. (A) Absolute numbers of MK cells (GPIIb/IIIa⁺) cells in MK cultures from fetal liver cells. Data shown are the mean ± SD from replicate cultures of LRP6^+/+ (n = 5) and LRP6^−/− (n = 4) cells. (B-C) Representative images from LRP6^+/+ and LRP6^−/− cultures, respectively. (D) Ploidy analysis of MKs derived from LRP6^+/+ and LRP6^−/− cultures. Data represent mean ± SD from replicate cultures of LRP6^+/+ (n = 5) and LRP6^−/− (n = 3) cells.