C/EBPα-p30 expression is correlated with increased miR-181a expression. (A) Overall Survival of younger (18-59 years) CN-AML patients with CEBPA wild-type, single mutations (N or C-terminal), or double mutations (N and C-terminal mutations together). (B) miR-181a expression in younger (18-59 years) CN-AML patients with either CEBPA wild-type (CEBPA wt), monoallelic C-terminal mutations (C-term. mut.), monoallelic N-terminal mutations (N-term. mut.), or concurrent N and C-terminal mutations (N+C term. mut.). (C) Representative patient bone marrow samples with characterized CEBPA mutations effecting either the N-terminus or C-terminus of the C/EBPα protein. Quantitative real-time RT-PCR data showing the association of the CEBPA mutation status and expression of the precursor (pre) miR-181a-1 (encoded on chromosome 1 locus) or miR-181a-2 (encoded on chromosome 9 locus). Each bar represents the average of triplicate measurements and error bars denote SEM.