Prolonged effect of N8-GP in a FeCl3-induced injury model in FVIII-deficient mice. Occlusion time after FeCl₃-induced injury was measured 5 minutes (acute effect), 24 hours, 48 hours, 60 hours, 72 hours, and 96 hours after dosing of 280 U/kg N8-GP (open circles) or FVIII (Advate; closed circles). The dotted line indicates the observation period for the experiments; during this period, no occlusion was recorded in the vehicle-treated mice. Mean and SEM of n = 6 to 10 mice per group are shown. *P < .01; **P < .05 N8-GP compared with FVIII at individual time points.