Prolonged effect of N8-GP in a tail bleeding model in FVIII-deficient mice. The mice were dosed intravenously with 200 U/kg of N8-GP (open bars) or FVIII (Advate, solid bars), and total bleeding time (A) and blood loss (B) were measured in the tail bleeding model after 5 minutes and 1, 2, or 3 days after treatment. The gray bars correspond to FVIII-deficient mice, and the hatched bars to normal mice (C57BL/6). Data are mean ± SEM of n = 6 to 12 mice per group. ***P < .001 indicates significant difference from FVIII-deficient mice. *P < .05 and **P < .01 indicate significant differences between mice treated with FVIII and N8-GP.