Reduced LRP binding of N8-GP. LRP was immobilized to a CM5 chip in a Biacore T100 instrument to 4000 to 5000 resonance units (RUs), and (A) FVIII (turoctocog alfa), (B) N8-GP, or (C) receptor-associated protein (RAP) at 12.5 nM, 3.13 nM, 0.78 nM, and 0.2 nM allowed to associate for 400 seconds and dissociate for 600 seconds using a flow of 10 µL/min.