A model of human PIEZO1. The predicted transmembrane regions of PIEZO1 (UniProt accession Q92508) were displayed using TMRPres2D software.²⁶  UniProt predictions are based on the regions that have α-helical transmembrane potential in at least 2 of the predictive tools, TMHMM, Memsat, Phobius, and the hydrophobic moment plot method of Eisenberg et al.²⁸  Arrows indicate the location of HX-associated mutations; red line, the location of PIEZO1 peptides identified in erythrocyte membranes; and green, the locations of peptides identified in discovery proteomics of erythrocyte membrane ghosts.