FGFR1 inactivation impairs HSPC mobilization induced by BM damage. (A). Flow cytometric analyses of the LSK population in PB and/or comparison of absolute numbers of LSKs/mL in PB between Mx1:Control, Mx1:CKO at homeostasis and Mx1:Control, Mx1:CKO (n = 4), Scl:Control, Scl:CKO (n = 3), Tek:Control, Tek:CKO (n = 4) mice 12 days after 5FU. (B) Comparison of total CFUs from PB of Mx1:Control, Mx1:CKO, mice at 14 days after 5FU (n = 4). (C) PB mononuclear cells 6 × 105) from Tek:Control, Tek:CKO mice (CD45.2) transplanted 12 days after 5FU together with 2 × 105 rescue BM cells (CD45.1) into lethally irradiated Ptprc recipients PB analysis for total engrafted donor cells at 4, 8, 12, and 16 weeks posttransplantation (n = 5). (D) Flow cytometric analyses of the LSK population in PB and comparison of absolute numbers of LSKs/mL PB between of Mx1:Control, Mx1:CKO, Scl:Control, and Scl:CKO mice at 1 hour after AMD3100 treatment (n = 3).
