FGF signal is activated post BM damage. (A) RNA-seq analysis of HSPCs for Fgfrs. Expression level shown by FPKM (fragments per kilobase of exon per million fragments mapped). (B) Gene expression analysis of Fgfr1, 2, 3, 4 using qRT-PCR on BM CD34⁻LSK cells from C57Bl/6 WT mice 5 days after 5FU (n = 3). (C) Gene expression analysis of Fgf1, 2, 3, 4, and 10 using qRT-PCR on BM cells from C57Bl/6 WT mice on the days indicated after 5FU (n = 3). (D) FGF1 and FGF2 protein levels as determined by ELISA of BM supernatants of C57Bl/6 WT mice after 5FU at 5 and 10 days, respectively (n = 3).