GATA-3-deficiency does not influence the survival of DN2 T-cell precursors. (A) The graphs show flow cyotmetric analysis of forward scatter, c-Kit, and IL-7Rα expression on CD44⁺CD25⁺CD4^-CD8^- “double negative 2” (DN2) subsets of Gata3^+/− and Gata3^−/− OP9Δ1 cocultures after 2 weeks. Filled histograms indicate Gata3^+/− cells, and solid lines indicate Gata3^−/− cells. DN4 cells served as controls (light gray histograms). (B) DN2 cells were analyzed for annexin V binding (apoptosis) and DiOC₆ staining. Filled histograms indicate Gata3^+/− cells, and solid lines indicate Gata3^−/− cells. PI⁺ cells were used as controls (light gray histograms). (C) DN1 and DN2 cells were analyzed for intracellular Bcl2 expression. Filled histograms indicate Gata3^+/− cells, and solid lines indicate Gata3^−/− cells. Lin^-CD25⁺ cells were used as a negative control (light gray histogram).