Proangiogenic capacity of CD34⁺ cells and capillary formation is increased by increasing miR-126 levels in high-glucose–treated CD34⁺ cells. (A) High-glucose–treated CD34⁺ cells were transfected with scrambled (□) and miR-mimic-126 (■) and the effects on endothelial cell tube formation are shown. (B) Representative photographs of tube formation in high-power field (n = 4). (C) MiR-126 levels in high-glucose–treated (HG) endothelial cells after treatment with healthy CD34⁺ cell supernatant. (D) Effect of direct exposure of high-glucose–treated HAECs to 25pmol scrambled-miR (□) or miR-mimic-126 (■) on tube-formation is shown (n = 5). (E) Expression levels of PI3KR2 in high-glucose–treated HAECs treated with 25pmol scrambled-miR (□) or miR-mimic-126 (■; n = 3-5). (F) Capillaries in the plug were assessed by staining for CD31 (green) and DAPI (blue) in in vivo Matrigel plug assay in diabetic mice (streptozotocin-induced) after administration of miR-mimic-126 or scrambled miR; a representative image is shown. (G) Hemoglobin content was assessed in the subcutaneously implanted Matrigel plugs of animals receiving scrambled or miR-mimic-126 (directly into plugs; n = 4). Data are mean ± SEM.