High-glucose exposure/diabetes leads to loss of proangiogenic effects and miR-126 expression and release from CD34⁺ PBMCs. (A) Effect of high-glucose 25mM (black) treatment compared with the normal glucose-treated CD34⁺ cells (n = 5-10). (B) Representative photographs of tube formation in high-power field. (C) Supernatant of high-glucose (■) treated cells as opposed to normal glucose (□) their effect on tube formation (n = 6-8). (D) Photographs of tube formation in high-power field. (E) Assessment of miR-126 in high-glucose–treated healthy CD34⁺ cells compared with untreated CD34⁺ cells (n = 4). (F) Assessment of the miR-126 levels in patients with type 2 diabetes (■) CD34⁺ cells in comparison with healthy subjects (□; n = 3). HG indicates high glucose; and NG, normal glucose. Data are mean ± SEM.