The methylome in APL. (A) Unsupervised hierarchical clustering based on raw DNA methylation levels. The color grid beneath visualizes the sample characteristics. The clustering is based on raw RRBS methylation values. (B) Distribution of methylation levels in APL and control samples. The stacked bar plots show the distribution of raw RRBS methylation levels of CpG sites covered in all samples for APL and control samples, respectively. The raw methylation levels (middle) are encoded by colors ranging from green (low methylation close to 0) to red (high methylation close to 1). Methylation levels were increased in APL samples (Kolmogorov-Smirnov test: P < .001). (C) Chromosomal distribution of covered CpG-clusters and DMRs. The plot shows the genome-wide distribution of CpG sites covered by RRBS together with DMRs detected between APL and controls. (D) Smoothed scatter plot of methylation values for APL versus healthy control samples. Colors represent the density of points ranging from red (high density) to blue (low density). Smoothed RRBS methylation levels were averaged for APL cells and controls. (E) Example of a differentially methylated region. The figure visualizes raw RRBS methylation levels for 2 exemplary samples (APL sample 10 and CD34 sample A) together with the estimated methylation levels for all APL and control samples at the CACNA1B promoter.