KPT-SINE significantly inhibits proliferation and induces cell-cycle arrest and apoptosis of AML cell lines and primary AML blasts. (A) WST-1 assays in MV4-11, Kasumi-1, MOLM-13, and OCI-AML-3. (B) Cell-cycle assessment using propidium iodine (PI) detection by flow cytometry at 24 hours. (C) Apoptosis as measured by annexin V/PI staining using FACS at 48 hours. (D) Colony assays in Kasumi-1, MV4-11, and OCI-AML3 cells after KPT-185 treatment at 24, 48, and 72 hours. (E) Apoptosis as measured by annexin V/PI staining using FACS at 48 hours in primary AML samples. (F) WST-1 assays in primary AML samples.