Fcγ receptor expression of slanDCs. (A) FcγR expression pattern of CD1c⁺ DCs, slanDCs, monocytes, and pDCs obtained through staining of PBMCs from buffy coats and subsequent flow cytometric analysis. Light gray and open histograms show control staining. Dark gray histograms refer to the mAb as indicated. Data are representative for 5 experiments. (B) Flow cytometric analysis of CD16 expression on slanDCs and neutrophils in PNH. Staining was performed with a mAb binding to both CD16a and CD16b (clone 3G8). The left panel shows CD16 control staining of slanDCs of a healthy donor. Open histogram: isotype control. Dark gray histogram: CD16-specific staining. The right panel shows CD16 expression on slanDCs and neutrophils of a healthy donor (open histograms) compared with a patient with PNH (gray histograms). (C) Freshly isolated and cultured PBMCs were stained for slanDCs and CD16, CD32, CD83, CD86, and HLA-DR to visualize the kinetics of FcγRs and maturation markers during maturation. Expression of any marker at the different time points was normalized to the isotype control and the sample with the highest expression. Data represent mean ± SEM (N = 5).