Activation of eNOS and Akt induced by ICAM-1 crosslinking. (A) In transfected HUVECs, ICAM-1 crosslinking (XL) induced the phosphorylation of eNOS at S1177 and Akt at S473, which were detected using phospho-specific antibodies. (B) Effect of Src inhibitor PP2 (10μM) and PI3K inhibitor wortmannin (Wort, 100nM) on the phosphorylation of ICAM-1, Src, Akt, and eNOS. Phosphorylation of ICAM-1, Src, and Cav-1 were sensitive to PP2, whereas the phosphorylation of Akt and eNOS was sensitive to both PP2 and wortmannin. *P < .05 versus all others.