Targeting of EglN genes corrects anemia related to inflammation. (A) Overview of rat anemia of inflammation model and dosing schedule. Rats were dosed with PG-APS polymer or PBS on day 0. Rats treated with PG-APS developed anemia by day 21 and were then randomized to receive LNPs targeting both EglN1 and EglN2, LNPs targeting firefly luciferase (LUC), or not treated (PG-APS only). (B-E) Hepatic mRNAs levels at termination of study on day 37. mRNA levels were normalized to GAPDH levels and then to the corresponding value for mice that received PBS instead of PG-APS. (F-G) Hemoglobin (F) and hematocrit (G) values for rats treated with the indicated siRNAs as in panel A. n = 4 except for PBS (n = 3). Error bars represent 1 SD. *P < .05; **P < .01.