Targeting of EglN genes rescues anemia caused by renal failure. (A) Overview of 5/6 nephrectomy procedure and dosing schedule. (B-F) mRNA values at day 12 in mice treated with the indicated siRNAs as depicted in panel A. HAMP1 indicates hepcidin antimicrobial peptide 1. mRNA levels were normalized to actin mRNA and then to the corresponding sham mRNA level. Sham mice underwent sham surgery rather than 5/6 nephrectomy. (G-I) Baseline hematocrit (day 0; G), day 12 hematocrit (H), and day 12 hematology parameters (I) in mice treated with the indicated siRNAs as depicted in panel A (n = 5). Error bars represent 1 SD. *P < .05; **P < .01.