Generation of a large T-NHL gene-expression profiling dataset and transcriptional characterization of ALCL patients. (A) Gene-expression data were extracted from the indicated datasets and normalized with robust multiarray average procedure. Samples classified as outlier on standard quality control analyses were removed from following computations. Expression of TNFRSF8 and/or ALK over the 25th percentile was required to include ALCL cases with higher neoplastic cell content. (B) Heat map of the top 50 genes up-regulated in ALK⁺ and ALK⁻ ALCL cases (30 ALK⁺ and 24 ALK⁻). Each colored cell in the heat map represents the gene-expression value for a probe in a sample. The highest expression values are displayed in red, the lowest values in blue.