Lymphocyte trafficking in St3gal4-deficient, St3gal6-deficient, and double-deficient mice. (A) ALNs, ILNs, MLNs, PPs, spleen (SPL), thymus (THY), and BM aggregates were isolated from each genotype. Lymphocytes recovered from each organ were quantified manually using a hematocytometer. All values are presented as mean ± SEM (n = 6). ***P < .001; **P < .01; *P < .05. (B) CFDA-labeled lymphocytes (2.5 × 107) obtained from wild-type MLNs were injected into the tail vein of recipient mice of the indicated genotypes. Lymphoid aggregates were harvested 1 hour after injection, and CFDA+ B lymphocytes (B220+) and T lymphocytes (CD3ϵ+) were quantified by flow cytometry. All values are presented as means ± SEM (n = 6). ***P < .001; **P < .01; *P < .05.
