IL1RAP knockdown decreases clonogenic potential and leads to increased cell death of AML cells. (A) Colony formation assays in semisolid methylcellulose media of AML cells (THP-1, OCI-AML3, HEL, and HL-60) infected with control and 2 IL1RAP-directed shRNAs. Data are mean ± SD. *P < .05. (B) Analysis of apoptosis/necrosis with annexin V/DAPI in THP-1 cells infected with control and IL1RAP shRNAs. Top panel: FACS contour plots of 1 representative experiment. Bottom panel: Mean ± SD of 3 independent experiments. *P < .05.