Golgi redistribution during thrombogenesis. (A) Immunofluorescence micrographs of immature and proplatelet-producing megakaryocytes showing Golgi remodeling into condensed vesicles that move into proplatelets during megakaryocyte maturation. Tubulin is visualized with an antitubulin mAb (red); nuclei by DAPI staining (blue); Golgi by anti-GM130 Abs (green). The micrographs are representative of > 10 experiments. Scale bar indicates10 μm. (B-C) Location of GM130 in proplatelets. Golgi is fragmented and condensed into small vesicular structures (green). Scale bar indicates 10 μm. (D) Kinetics of the Golgi disassembly in a mouse megakaryocyte transfected with the Golgi marker GalNAc-T2-stem-YFP. Panels of micrographs selected from 77 minutes of real-time observation. (E) Double immunofluorescence microscopy of transfected megakaryocyte with GalNAc-T2-stem-YFP using anti-YFP (green) and anti-GM130 (red) Ab. Colocalization was seen in cells expressing GalNAc-T2-stem-YFP regardless of the maturation level (not shown). (F) Immunofluorescence microscopy of differentiated megakaryocytes using the Golgi marker anti-GM130 Ab (green) and the endoplasmic reticulum (ER) marker Calnexin (red). (G-H) Arrow shows GM130 accumulation in a proplatelet swelling. Between swellings, small GM130-containing vesicles are stained. (I) Electron micrograph of a section of a proplatelet extension/swelling containing a small Golgi-like vesicular stack (arrow).