Gaussian kernel density plots indicating the frequency of cells carrying all acquired mutations. Frequency is calculated by adjusting mutant allele burden by copy number of the loci mutated. Samples with mutated KRAS (A), NRAS (B), or BRAF (C) indicates that driver mutations may be in major (> 0.5) or minor (< 0.5) clone populations. Frequency of the mutated RAS pathway gene is indicated by a dotted line.