Positions, sequences, and ranking of epitopes derived from the mutational region of NPM1. The entire amino acid sequences of the NPM1 wild-type protein and of the mutated cytoplasmic NPM1 types A, B, C, and D were screened for HLA-A*0201–binding T-cell epitopes using several software programs. The ten 9 mer peptides NPM1#1 to NPM1#9 and wild-type with the highest scores for human HLA-A*0201 binding were used for further experiments.