Detection of plasminogen bound to the surfaces of normal peripheral blood cells. (A) Isolated peripheral blood cells were preincubated with either 10μM plasminogen (black tracings) or buffer (blue tracings) and analyzed in FACS analyses with mAb49 as described in “Methods.” (B) Isolated neutrophils were incubated with 20μM plasminogen (black tracing), autologous plasma (green tracing), or buffer (blue tracing) and analyzed in FACS with mAb49. In addition, FACS analyses were performed with mAb49 in whole blood (red tracing), gated for neutrophils using CD15 as described in “Methods.” (C) FACS analyses was performed with mAb49 (red tracing) or isotype control (purple tracing) in whole blood gated for the indicated cell types: CD15 for neutrophils, CD14 for monocytes, CD2 for T lymphocytes, and CD19 for B lymphocytes. (D) FACS analyses were performed with mAb49 (red tracing) or isotype control (purple tracing) in whole blood gated for platelets with anti–GPIIb-IIIa mAb.