Technical performance of immune repertoire sequencing assay. Diagnostic samples from 12 ALL patients containing 13 leukemic IgH clonotypes were used for technical performance studies. Serial dilutions of leukemic cells in normal PBMCs, ranging from < 1 in 1 million to > 1 in 1000 tumor cells, were prepared and analyzed in duplicate. The 7 duplicated dilutions were then subjected to amplification and sequencing in 2 replicate experiments. The expected and observed frequencies were compared on a logarithmic scale. Replicate 1 is represented by circles and replicate 2 is represented by crosses in all panels. Panel G shows the results from 2 cancer clones that were present in this tumor.