The TR47 peptide and TRAP are PAR1 biased agonists. Differential phosphorylation was determined for pThr202/Tyr204-ERK1/2, pSer473-Akt, and pSer9-GSK3β that was induced by TRAP, TR47 peptide, control scrTR47 peptide, APC, and thrombin. (A) Time course of ERK1/2 phosphorylation at Thr202/Tyr204 by APC (70nM) and TR47 (50μM). (B) Phosphorylation of ERK1/2 at Thr202/Tyr204 by TRAP (50μM), TR47 (50μM), scrTR47 (50μM), and APC (70nM) over time. (C) Time course of Akt phosphorylation at Ser473 by APC (70nM) and thrombin (54nM). (D) Phosphorylation of Akt at Ser473 by APC (70nM), thrombin (54nM), and TRAP (50μM) over time. (E) Time course of GSK3β phosphorylation at Ser9 by APC (70nM) and TRAP (50μM). (F) Phosphorylation of GSK3β at Ser9 by APC (70nM) and TRAP (50μM) over time. (A,C,E) Representative experiments. (B,D,F) Data points represent the mean ± SEM (N ≥ 3).