American Society of Hematology

Figure 1

$Figure 1. Ribosomal profiles are altered in SDS patient cells. (A) Bone marrow stromal cell lysates from healthy controls or SDS patients were fractionated through 7%-47% sucrose gradients by ultracentrifugation. Representative polysome profiles are shown. Relative 80S:40S ratios were quantified for 3 healthy controls versus 5 SDS patients (DF259, FHCRC18, FHCRC30, FHCRC32, UW1) of different SBDS genotypes (listed in supplemental Table 1). (B) Ribosomes were dissociated with 0.5M KCl or 0.25mM MgCl2 into 40S and 60S subunits, followed by sucrose density centrifugation. Representative polysome gradients are shown. 60S:40S ratios in 0.5M KCl were quantified for 3 healthy controls versus 3 SDS patients (DF259, FHCRC32, UW1) and the aggregate results are graphed; *P < .05. For 2 controls and 2 patients, the assay was repeated up to 3 times with consistent results (methods to quantitate the 40S, 60S, and 80S peaks are illustrated in supplemental Figure 1).$

Ribosomal profiles are altered in SDS patient cells. (A) Bone marrow stromal cell lysates from healthy controls or SDS patients were fractionated through 7%-47% sucrose gradients by ultracentrifugation. Representative polysome profiles are shown. Relative 80S:40S ratios were quantified for 3 healthy controls versus 5 SDS patients (DF259, FHCRC18, FHCRC30, FHCRC32, UW1) of different SBDS genotypes (listed in supplemental Table 1). (B) Ribosomes were dissociated with 0.5M KCl or 0.25mM MgCl₂ into 40S and 60S subunits, followed by sucrose density centrifugation. Representative polysome gradients are shown. 60S:40S ratios in 0.5M KCl were quantified for 3 healthy controls versus 3 SDS patients (DF259, FHCRC32, UW1) and the aggregate results are graphed; *P < .05. For 2 controls and 2 patients, the assay was repeated up to 3 times with consistent results (methods to quantitate the 40S, 60S, and 80S peaks are illustrated in supplemental Figure 1).

This Feature Is Available To Subscribers Only