Expression of ANXA2 and validation of gene expression by flow cytometry and Western blotting. (A) Expression of ANXA2 and its receptor, AX2R, in memory B cells (MBCs), polyclonal plasmablastic cells (PPCs), normal plasma cells (BMPCs), myeloma cells (MMCs) and human myeloma cell lines (HMCLs). (B) To validate gene-expression data, the expression of ANXA2 was determined by flow cytometry. Light gray line indicates control without primary Ab; black line, measurement with the corresponding primary and secondary Abs. All cell lines show a consistent expression of ANXA2 by gene-expression profiling, real-time PCR, flow cytometry, and Western blotting (C). For Western blotting, β-actin was used as loading control.