ALL cells at diagnosis and relapse cluster to different lineages. (A) Patient L3 with B-cell acute lymphoblastic leukemia (B-ALL): (Left) Reconstructed lineage tree of CD34 blasts from peripheral blood (PB) individual leukemia cells at diagnosis (blue) and relapse (red) for patient L3. Root (black) was calculated from the median signal of all cells. (Right) Comparison of depth between cells at diagnosis (blue) and relapse (red; P|=|.91). (B) Patient L4 with B-ALL: (Left) Reconstructed lineage tree of CD2CD4CD3 blasts from PB individual leukemia blast cells at diagnosis (blue) and relapse (red) for patient L4. (Right) Comparison of depth between cells at diagnosis (blue) and relapse (red; P|=|.88). The most significant P value for clustering is indicated. The width of the colored branches represents the level of clustering significance (wider lower P value). Y-axis represents depth in arbitrary units.