Leukemia cells at diagnosis and relapse from 2 patients with AML. (A) Patient L1 with acute myeloid leukemia (AML). (Left) Reconstructed lineage tree of CD33⁺CD4⁺CD15⁻ blasts from peripheral blood (PB) individual leukemia cells at diagnosis (blue) and relapse (red). T cells (TCs) from PB both at diagnosis and relapse were CD3⁺CD33⁻CD4⁻ (green); root (black) was calculated from the median signal of all cells. (Right) Comparison of depth between cells at diagnosis (blue) and relapse (red; P < .0005). (B) Patient L2 with AML (Left) Reconstructed lineage tree of CD117⁺ blasts from PB individual leukemia blast cells at diagnosis (blue) and relapse (red). CD34⁺CD38⁻CD90⁺ leukemia stem cell (LSC)–enriched population from BM at diagnosis (orange). Root (black) was calculated from the median signal of all cells. (Right) Comparison of depth between cells at diagnosis (blue) and relapse (red; P = .002). The most significant P value for clustering is indicated. The width of the colored branches represents the level of clustering significance (wider = lower P value). Y-axis represents depth in arbitrary units. The numbers below the cells represent the FLT3/internal tandem duplication genotype of each cell only if duplication was present; wild types are blanks.