Figure 3
Figure 3. Basophils increase the proportion of IL-17+/IFN-γ+ and IL-17+/IFN-γ− but not IL-17−/ IFN-γ+ cells in TEM and TCM. TEM and TCM cells were cultured with or without basophils in the presence of IL-33. TEM were stimulated by IL-2 (A) and TCM were stimulated by anti-CD3/28–coated beads (B). After 5 days, cells were restimulated with PMA for 6 hours and IL-17 and IFN-γ expression was examined using intracytoplasmic staining. **P < .01.

Basophils increase the proportion of IL-17+/IFN-γ+ and IL-17+/IFN-γ but not IL-17/ IFN-γ+ cells in TEM and TCM. TEM and TCM cells were cultured with or without basophils in the presence of IL-33. TEM were stimulated by IL-2 (A) and TCM were stimulated by anti-CD3/28–coated beads (B). After 5 days, cells were restimulated with PMA for 6 hours and IL-17 and IFN-γ expression was examined using intracytoplasmic staining. **P < .01.

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