BDCA-1⁺ rather than BDCA-3⁺ DCs internalize immune-complexed antigen. Human BDCA-1⁺ and BDCA-3⁺ DCs were cultured in the presence of eGFP-IC for 10 minutes, washed 3 times, and incubated for 1 hour (37°C). Uptake was analyzed using flow cytometry (A-B). Shown are representative plots of eGFP-IC (black line) uptake (A) and mean uptake (percentage positive cells, left or MFI, right) of BDCA-1⁺ (white bars) and BDCA-3⁺ DCs (black bars).