Gene expression in Spi-B–deficient pDCs. (A) Expression profiles of WT and Spi-B–deficient BM pDCs. CD11c⁺B220⁺BST2⁺ cells from WT and Spi-B–deficient mice were sorted as BM pDCs and used. The scatter plot represents normalized log intensities of individual probes. The lines indicate the 3-fold difference. (B) Expression profiles of indicated genes in sorted BM pDCs were analyzed by quantitative real-time PCR. BM pDCs were pooled from 4 mice for each experiment. Data are representative of 2 independent experiments (means ± SD). (C) Clustering of cell populations by probe sets with expression that was more than 10-fold down-regulated in Spi-B–deficient BM pDCs. The results were compared with the expression database of normal immune cell populations (Gene Expression Omnibus dataset GSE9810).