Isolation of HY tetramer^pos T cells from female UCB. (A) Example of gate setting for FACS sorting to collect all tetramer^pos cells. Gate settings were based on tetramer staining intensity of a well-described HY^A2 or HY^B7 tetramer^pos CTL clone.²¹  (B) Representative example of CD45RO expression of CD8^posHY^A2tetramer^pos collected T cells.